| 范浩浩；董宏利.吴门医派验方通窍止痛方对偏头痛模型大鼠的改善作用及机制研究[J].中医药信息,2024,41(5):13-18 |
| 吴门医派验方通窍止痛方对偏头痛模型大鼠的改善作用及机制研究 |
| Improvement Effect and Mechanism of Wumen Proved Prescription Tongqiao Zhitong Formula on Migraine Model Rats |
| 投稿时间：2023-07-24 录用日期：2023-08-25 |
| DOI：10.19656/j.cnki.1002-2406.20240503 |
| 中文关键词: 偏头痛 通窍止痛方 大鼠 行为学 作用机制 |
| 英文关键词: Migraine Tongqiao Zhitong Formula Rats Behavior Mechanism of action |
| 基金项目:苏州市姑苏卫生重点人才项目(GSWS2020080)；第七批全国老中医药专家学术经验继承人项目。 |
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| 中文摘要: |
| 目的：通过观察吴门医派验方通窍止痛方对偏头痛大鼠模型血清一氧化氮（NO）、降钙素基因相关肽（CGRP）、内皮素-1(ET-1)、神经递质多巴胺（DA）、去甲肾上腺素（NE）及5-羟色胺（5-HT）含量的影响探讨其作用机制。方法：将SD大鼠随机分为空白组、模型组、西比灵组和通窍止痛方组，每组8只，连续灌胃给予相应药物7 d，末次灌胃30 min后，除空白组外，其余各组以10 mg/kg剂量在颈部皮下注射硝酸甘油（GTN）的方式制备偏头痛大鼠模型。观察大鼠2 h内（以30 min为间隔）挠头次数，记录其行为学变化；造模4 h后，采用酶联免疫吸附实验（ELISA）检测大鼠血清中NO、CGRP、ET-1及脑组织中DA、NE、5-HT水平。结果：与空白组比较，模型组大鼠不同时间段挠头次数显著增多（P <0.01），血清中NO、CGRP水平显著升高（P <0.01）,ET-1水平明显下降（P <0.01）；脑组织中DA、NE、5-HT水平均显著降低（P <0.01）；与模型组比较，西比灵组、通窍止痛方组大鼠不同时间段挠头次数均显著降低（P <0.05,P <0.01），血清中NO、CGRP水平显著降低（P <0.01,P <0.05）,ET-1水平显著升高（P <0.05,P <0.01）；脑组织中DA、NE、5-HT水平明显升高（P <0.01,P <0.05）。结论：通窍止痛方对偏头痛大鼠模型的改善作用可能与调节血清NO、CGRP、ET-1水平及脑组织神经递质DA、NE、5-HT含量有关。 |
| 英文摘要: |
| Objective: To investigate the effects of Wumen proved prescription Tongqiao Zhitong Formula(TZF) on migraine model rat serum nitric oxide(NO), calcitonin gene-related peptide(CGRP), endothelin-1(ET-1), neurotransmitter dopamine(DA), norepinephrine(NE), and serotonin(5-HT), and its mechanism. Methods: SD rats were randomly assigned into blank group,model group,flunarizine group,and TZF group,with 8 rats in each group,and the rats were gavaged once a day for 7 days. Thirty min after the last gavage, the migraine rat model was prepared by subcutaneous injection of glyceryl trinitrate(GTN) into the neck at the dose of 10 mg/kg in all groups except the blank group. The number of scratching of the head within 2 h(30 min interval) was observed, and the behavioral changes were recorded. The levels of NO, CGRP and ET-1 in serum and DA, NE and 5-HT in brain tissue were detected by ELISA 4 h after modeling. Results: Compared with the blank group,the number of head scratches in each time period of rats in the model group significantly increased(P < 0. 01), the levels of NO and CGRP in serum significantly increased(P < 0. 01), and the level of ET-1 was significantly decreased(P < 0. 01). The levels of DA,NE and 5-HT in brain tissues were significantly reduced(P < 0. 01). Compared with the model group,in flunarizine group and TZF group, the number of head scratches in each time period was significantly reduced(P < 0. 05 or P < 0. 01),the levels of NO and CGRP in serum decreased(P < 0. 05 or P < 0. 01), the level of ET-1 were significantly increased(P < 0. 05 or P < 0. 01), and the levels of DA,NE and 5-HT in brain tissue were significantly increased(P < 0. 01 or P < 0. 05). Conclusion: The improvement effect of Tongqiao Zhitong Formula on migraine rat model may be related to the regulation of serum NO,CGRP,ET-1 and the content of brain tissue neurotransmitters DA,NE and 5-HT. |
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