高氏盆炎方一号方对慢性盆腔炎模型大鼠抗炎作用及其对TGF－β1、MMP－2的影响

侯金萌1; 郝艳红2; 徐文君3; 刘玉兰2; 高慧2*; 张晴1; 龙森2; 吴松柏

文章摘要

侯金萌1，郝艳红2，徐文君3，刘玉兰2，高慧2*，张晴1，龙森2，吴松柏.高氏盆炎方一号方对慢性盆腔炎模型大鼠抗炎作用及其对TGF－β1、MMP－2的影响[J].中医药信息,2025,42(4):12-18

高氏盆炎方一号方对慢性盆腔炎模型大鼠抗炎作用及其对TGF－β1、MMP－2的影响

Anti－Inflammatory Effect of Gao's Pelvic Inflammation Formula No. 1 on Chronic Pelvic Inflammation Model Rats and Its Influence on TGF－β1 and MMP－2

投稿时间：2024-10-23 录用日期：2024-11-19

DOI：10.19656/j.cnki.1002-2406.20250402

中文关键词: 高氏盆炎方一号方慢性盆腔炎转化生长因子－β1 基质金属蛋白酶－2 抗炎抗粘连

英文关键词: Gao's Pelvic Inflammation Formula No. 1 Chronic pelvic inflammation Transforming growth factor－β1 Matrix metalloproteinase－2 Anti－inflammatory Anti－adhesion

基金项目:国家中医药管理局全国名老中医药专家高慧传承工作室建设项目

作者	单位
侯金萌1，郝艳红2，徐文君3，刘玉兰2，高慧2*，张晴1，龙森2，吴松柏

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中文摘要:

目的：探究高氏盆炎方一号方对慢性盆腔炎模型大鼠TGF－β1、MMP－2细胞因子的影响及其抗炎、抗粘连作用机制。方法：75只12周龄SD雌性大鼠分为空白组10只和造模组65只，造模组采用25%苯酚凝胶化学烧伤联合机械损伤法建立盆腔炎大鼠模型。造模结束后10 d，从造模组中随机抽取5只大鼠，进行解剖，观察成模情况。造模组剩余60只大鼠，随机分为模型组、蒲苓组、胎盘注射液组和中药高、中、低剂量组各10只。模型组给予生理盐水2 mL/（100g∙d）灌胃；中药低、中、高剂量组分别给予高氏盆炎方一号方7、14、28 g/（kg∙d）灌胃；蒲苓组给予蒲苓盆炎康颗粒3 g/（kg∙d）灌胃；胎盘注射液组给予人胎盘组织液1 mL/（kg∙d）肌内注射，连续给药20 d。光镜下观察大鼠子宫组织形态学变化，ELISA法检测大鼠血清TGF－β1、IL－10水平，免疫组化法检测子宫组织TGF－β1、MMP－2蛋白的表达情况。结果：光镜下观察模型组大鼠子宫管壁增厚，内膜充血水肿，组织结构错乱，腺体变形，可见炎细胞浸润，上皮细胞变性坏死，成纤维细胞增生，提示造模成功。ELISA法检测大鼠血清TGF－β1、IL－10水平：与其他各组比较，模型组大鼠血清IL－10浓度降低，TGF－β1水平升高，差异有统计学意义（P < 0.05）。与模型组比较，中药高、中、低剂量组及胎盘注射液组、蒲苓组IL－10的水平升高，TGF－β1水平降低（P < 0.05）。中药高、中剂量组与空白组及胎盘注射液组比较，差异无统计学意义（P > 0.05）。免疫组化法检测大鼠子宫中MMP－2、TGF－β1蛋白表达：与其他各组比较，模型组MMP－2的水平降低，TGF－β1水平升高，差异有统计学意义（P < 0.05）。与模型组比较，中药高、中、低剂量组MMP－2的表达升高，TGF－β1表达降低，差异有统计学意义（P < 0.05），中药高、中剂量组与空白组相及胎盘注射液组比较，差异无统计学意义（P > 0.05），中药高、中剂量组与蒲苓组比较，差异有统计学意义（P < 0.05）。结论：采用苯酚凝胶化学烧伤联合机械损伤法制备大鼠慢性盆腔炎模型复制成功，经高氏盆炎方一号方治疗后，大鼠子宫组织得以修复，炎症反应及纤维化减轻，防止粘连，其作用机制与高氏盆炎方一号方升高炎症及粘连相关因子IL－10、MMP－2水平，降低炎症及粘连细胞因子TGF－β1的水平相关。

英文摘要:

Objective： To explore the anti－inflammatory and anti－adhesion mechanisms of Gao's pelvic inflammation formula No. 1 in chronic pelvic inflammation model rats and its effect on the cytokines TGF－β1 and MMP－2. Methods： A total of 75 female SD rats （12 weeks old） were divided into a blank group （10 rats） and a model group （65 rats）. The pelvic inflammation model was established using a 25% phenol gel chemical burn combined with mechanical injury. Ten days after modeling， five rats from the model group were randomly selected for dissection to observe the modeling condition. The remaining 60 rats were randomly assigned into the model group， the Puling group， the placenta injection group， and the high， medium， and low dose Chinese medicine groups （each with 10 rats）. The model group was given physiological saline 2 mL/（100g∙d） by gavage； the Chinese medicine groups were given Gao's Pelvic Inflammation Formula No. 1 at doses of 7， 14， and 28 g/（kg∙d）； the Puling group was given Puling Pelvic Inflammation Granules 3 g/（kg∙d） by gavage； the placenta injection group received human placenta tissue fluid 1 mL/（kg∙d） by intramuscular injection， continuously for 20 days. Light microscopy was used to observe the uterine tissue morphology， ELISA was used to detect serum levels of TGF－β1 and IL－10， and immunohistochemistry was used to detect the expression of TGF－β1 and MMP－2 proteins in uterine tissue. Results： Light microscopy showed that the uterine walls in the model group rats thickened， the endometrial tissue was congested and edematous， the tissue structure was disordered， the glands were deformed， and inflammatory cell infiltration was observed， indicating successful modeling. ELISA detection of serum TGF－β1 and IL－10 levels showed that compared to other groups， the model group had significantly reduced IL－10 and elevated TGF－β1 levels （P < 0.05）. Compared to the model group， the Chinese medicine groups （high， medium， and low doses）， the placenta injection group， and the Puling group all had elevated IL－10 and decreased TGF－β1 levels （P < 0.05）. No significant differences were observed between the high and medium dose Chinese medicine groups， the blank group， and the placenta injection group （P > 0.05）. Immunohistochemistry showed that the model group had decreased MMP－2 and increased TGF－β1 expression levels （P < 0.05）. Compared to the model group， the Chinese medicine groups （high， medium， and low doses） showed increased MMP－2 expression and decreased TGF－β1 expression （P < 0.05）. There was no significant difference between the high and medium dose groups， the blank group， and the placenta injection group （P > 0.05）， but significant differences were found between the high and medium dose Chinese medicine groups and the Puling group （P < 0.05）. Conclusion： The chronic pelvic inflammation model in rats was successfully replicated using phenol gel chemical burn combined with mechanical injury. After treatment with Gao's Pelvic Inflammation Formula No. 1， the uterine tissue was repaired， inflammation and fibrosis were alleviated， and adhesion was prevented. The mechanism of action involves Gao's Pelvic Inflammation Formula No. 1 increasing the inflammatory and adhesion－related factors IL－10 and MMP－2， while reducing the levels of inflammatory and adhesion cytokine TGF－β1.

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