基于Wnt/β－catenin信号通路探讨绿原酸对幽门螺杆菌相关性胃炎模型大鼠的抑菌作用及胃黏膜保护效应

吴迪1; 丁姣姣1; 王隆轩1; 张国斌

文章摘要

吴迪1，丁姣姣1，王隆轩1，张国斌.基于Wnt/β－catenin信号通路探讨绿原酸对幽门螺杆菌相关性胃炎模型大鼠的抑菌作用及胃黏膜保护效应[J].中医药信息,2025,42(4):24-28

基于Wnt/β－catenin信号通路探讨绿原酸对幽门螺杆菌相关性胃炎模型大鼠的抑菌作用及胃黏膜保护效应

Bacteriostasis and Gastric Mucosal Protective Effects of Chlorogenic Acid in Helicobacter pylori－Associated Gastritis Rats Based on the Wnt/β－Catenin Signaling Pathway

投稿时间：2024-10-27 录用日期：2024-12-11

DOI：10.19656/j.cnki.1002-2406.20250404

英文关键词: Chlorogenic acid Helicobacter pylori－associated gastritis Interleukin 8 Tumor necrosis factor α Wnt/β－catenin signaling pathway

基金项目:

作者	单位
吴迪1，丁姣姣1，王隆轩1，张国斌

摘要点击次数: 53

全文下载次数: 34

中文摘要:

目的：基于Wnt/β－catenin信号通路研究绿原酸对幽门螺杆菌相关性胃炎大鼠的治疗作用，探讨其作用机制。方法：采用幽门螺杆菌联合化学致癌剂MNNG诱导幽门螺杆菌胃炎大鼠模型，造模成功后分为模型组、阳性组、绿原酸高剂量组与绿原酸低剂量组，分别灌胃等体积溶媒、铋剂四联药物、100 mg/kg绿原酸与50 mg/kg绿原酸，另设正常组，每组10只，共治疗4周。采用快速尿素酶试验评价各组胃炎大鼠胃组织中幽门螺杆菌的定植程度，光学显微镜观察胃黏膜组织病理学，酶联免疫吸附试验测定血清IL－8、TNF－α等炎症细胞因子含量，RT－qPCR试验检测胃黏膜Wnt2、β－catenin基因表达。结果：与模型组相比，绿原酸高、低剂量组胃黏膜的充血水肿、腺体排列及炎性细胞浸润情况等均有所改善；绿原酸高、低剂量组幽门螺杆菌定植程度显著改善，幽门螺杆菌清除率分别为50%、33%（P 0.01）；绿原酸高、低剂量组大鼠血清IL－8、TNF－α含量明显降低（P 0.01），胃黏膜Wnt2、β－catenin相对基因表达量明显下调（P 0.01）；绿原酸高、低剂量组血清IL－8、TNF－α含量高于阳性组，胃黏膜Wnt2基因相对表达量则显著低于阳性组（P 0.01）。结论：绿原酸对幽门螺杆菌相关性胃炎大鼠具有治疗作用，可改善幽门螺杆菌定植程度、保护胃黏膜，其作用途径可能涉及抑制Wnt/β－catenin通路的异常活化，下调IL－8、TNF－α表达等环节。

英文摘要:

Objective： To investigate the therapeutic effects of chlorogenic acid on Helicobacter pylori （H. pylori）－associated gastritis in rats， based on the Wnt/β－catenin signaling pathway， and explore its mechanism of action.Methods： A rat model of H. pylori－induced gastritis was established using a combination of H. pylori and the chemical carcinogen MNNG. After successful modeling， the rats were divided into four groups： the model group， the positive control group， the high－dose chlorogenic acid group （100 mg/kg）， and the low－dose chlorogenic acid group （50 mg/kg）， with normal controls. Each group consisted of 10 rats， and the treatment lasted for 4 weeks. The degree of H. pylori colonization in the gastric tissue of each group was evaluated using the rapid urease test. Histopathological examination of the gastric mucosa was conducted by optical microscopy. The levels of inflammatory cytokines IL－8， TNF－α in serum were determined by enzyme－linked immunosorbent assay （ELISA）. The expression of Wnt2 and β－catenin genes in the gastric mucosa was detected by RT－qPCR.Results： Compared to the model group， the high and low－dose chlorogenic acid groups showed significant improvements in gastric mucosal congestion， edema， glandular arrangement， and inflammatory cell infiltration. The degree of H. pylori colonization was significantly reduced in both the high and low－dose chlorogenic acid groups， with eradication rates of 50% and 33%， respectively （P 0.01）. Serum levels of IL－8 and TNF－α in the high and low－dose chlorogenic acid groups were significantly lower （P 0.01）， and the relative gene expression of Wnt2 and β－catenin in the gastric mucosa was significantly downregulated （P 0.01）. The serum levels of IL－8 and TNF－α in the chlorogenic acid groups were higher than those in the positive control group， while the relative gene expression of Wnt2 in the gastric mucosa was significantly lower than in the positive control group （P 0.01）.Conclusion： Chlorogenic acid has therapeutic effects on H. pylori－associated gastritis in rats. It can improve H. pylori colonization and protect the gastric mucosa. The mechanism may involve inhibiting the abnormal activation of the Wnt/β－catenin pathway and downregulating the expression of IL－8 and TNF－α.

查看全文查看/发表评论下载PDF阅读器

关闭