| 汪旭,郝晓蓓,杨敏,潘娅岚,王庆,徐桂华.扶正补血食疗方含药血清干预Notch通路调控骨髓造血干细胞化疗后损伤的机制研究[J].中医药信息,0,(): |
| 扶正补血食疗方含药血清干预Notch通路调控骨髓造血干细胞化疗后损伤的机制研究 |
| Study on the Mechanism of Fuzheng Buxue Diet Therapy Recipe's Serum Intervening Notch Pathway to Regulate the Damage of Bone Marrow Hematopoietic Stem Cells After Chemotherapy |
| 投稿时间：2021-11-11 录用日期：2021-11-15 |
| DOI： |
| 中文关键词: Notch通路 化疗 中医食疗 共培养 造血干细胞 |
| 英文关键词: Notch pathway chemotherapy TCM diet therapy co-culture hematopoietic stem cells |
| 基金项目:江苏省卫生健康委医学科研项目指导性项目(Z2021065)；江苏省中医药科技发展计划项目（YB2020004）；江苏省研究生科研创新计划项目（KYCX21_1803）；江苏省研究生科研创新计划项目（SJCX21_0665） |
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| 中文摘要: |
| 目的：通过扶正补血食疗方含药血清干预5-FU损伤小鼠骨髓间充质干细胞（BMSCs）和造血干细胞（HSCs）体外共培养模型，探讨补血食疗方对化疗后模拟“造血龛”中小鼠HSCs增殖、凋亡以及Notch信号通路的影响。方法：建立小鼠BMSCs和HSCs的共培养模型。共培养细胞被随机分为3组: 空白组、5-FU组、扶正补血食疗方含药血清组。镜下观察各组细胞形态变化，取上层悬浮HSCs 进行检测，CCK-8检测细胞活力、PI/RNase法检测细胞周期、Annexin V/Alexa Fluor 647双重染色法检测细胞凋亡率、qRT-PCR检测Jagged1、Notch1、Notch2基因的表达水平。结果：与正常组相比，5-FU组细胞活性降低(P< 0.05)，G0/G1期细胞占比增加(P< 0.05)，凋亡率增加(P< 0.05)，在基因水平，Jagged1、Notch1、Notch2表达呈下调趋势(P< 0.05); 与5-FU组相比，扶正补血食疗方含药血清组细胞活性增加(P< 0.05)，G0/G1期细胞占比减少(P< 0.05)，总凋亡率呈下降趋势(P< 0.05)，在基因水平，Jagged1、Notch1、Notch2表达呈上调趋势(P< 0.05)。结论：扶正补血食疗方含药血清可能通过上调Jagged1、Notch1、Notch2基因的表达激活Notch通路，保护造血微环境，促进 HSCs增殖、降低凋亡率，缓解HSCs化疗损伤程度。 |
| 英文摘要: |
| Objective Through Fuzheng Buxue Food Therapy Recipe containing serum to intervene in 5-FU-injured mice bone marrow mesenchymal stem cells (BMSCs) and hematopoietic stem cells (HSCs) in vitro co-culture model, to explore the effect of Buxue Food Therapy on the proliferation of mouse HSCs in the "hematopoietic niche" after chemotherapy , Apoptosis and the influence of Notch signaling pathway. Methods Establish a co-culture model of mouse BMSCs and HSCs. The co-cultured cells were randomly divided into 3 groups: blank group, 5-FU group, and serum group containing Fuzheng Buxue Food Therapy. Observe the morphological changes of the cells in each group under the microscope, take the upper suspended HSCs for detection, CCK-8 to detect cell viability, PI/RNase method to detect cell cycle, Annexin V/Alexa Fluor 647 double staining method to detect cell apoptosis rate, qRT-P CR Detect the expression levels of Jagged1, Notch1, Notch2 genes. Results Compared with the normal group, the cell viability of the 5-FU group decreased (P<0.05), the proportion of cells in the G0/G1 phase increased (P<0.05), and the apoptosis rate increased (P<0.05). At the gene level, Jagged1, Notch1 The expression of Notch2 and Notch2 showed a down-regulation trend (P<0.05); Compared with the 5-FU group, the cell viability of the serum group containing Fuzheng Buxue Food Therapy Recipe increased (P<0.05), and the proportion of cells in the G0/G1 phase decreased (P<0.05) , The total apoptosis rate showed a downward trend (P<0.05). At the gene level, the expression of Jagged1, Notch1, and Notch2 showed an upward-regulated trend (P<0.05). Conclusion The serum of Fuzheng Buxue Food Therapy Prescription may activate the Notch pathway by up-regulating the expression of Jagged1, Notch1 and Notch2 genes, protect the hematopoietic microenvironment, promote HSCs proliferation, reduce the rate of apoptosis, and alleviate the degree of HSCs chemotherapy damage. |
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