文章摘要
基于“阳中求阴”理论研究左归丸对肾阴虚证能量代谢的影响
The effect of zuogui pill on energy metabolism of kidney Yin deficiency syndrome was studied based on the theory of "seeking Yin in Yang"
投稿时间:2020-04-21  录用日期:2020-05-19
DOI:
中文关键词: 线粒体  能量代谢  左归丸  阳中求阴
英文关键词: Mitochondria  Energy metabolism  Left to pill  Yang in Yin
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位邮编
李璐 甘肃中医药大学 730000
梁永林* 甘肃中医药大学 730000
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中文摘要:
      目的 通过观察左归丸及其拆方组对采用灌胃甲状腺素片与利血平混合悬 浮液制备的肾阴虚证大鼠肝线粒体能量代谢的影响。探讨左归丸“阳中求阴”治 疗肾阴虚证的作用机理。方法 采用灌胃甲状腺素片与利血平混合悬浮液制备肾 阴虚证模型,并随机分为模型组、左归丸组、拆方滋阴组、拆方补阳组,检测大 鼠肝组织线粒体中 SDH、Na+-K+-ATPase、CCO、LDH 活性及 ATP 含量变化,透射电 镜下观察肝组织中线粒体超微结构的变化。结果 与对照组比较,模型组大鼠肝 线粒体 SDH、Na+-K+-ATPase、CCO、LDH 活性及 ATP 含量均显著下降(P<0.01), 肝线粒体超微结构损伤严重;与模型组比较,左归丸组上述酶的活性及 ATP 含量 均显著升高(P<0.05 或 P<0.01),肝线粒体超微结构的病理变化明显改善。结 论 干预肝线粒体能量代谢障可能是左归丸“阳中求阴”治疗肾阴虚证的作用机 理,进一步体现左归丸“阳中求阴”的配伍意义。
英文摘要:
      Objective to observe the effect of zuogui pill and its prescription group on the energy metabolism of liver mitochondria in rats with kidney-yin deficiency syndrome prepared by gavage thyroxine tablets and rexepine mixture. Objective: to investigate the mechanism of zougui pill "Yang zhong Yin seeking" in the treatment of kidney Yin deficiency syndrome. Methods the model of kidney Yin deficiency syndrome was prepared by the mixture of gavage thyroxine tablets and rexuepin, and randomly divided into model group, zuo GUI pill group, fang ziyin group and fang buyang group. The changes of SDH, Na+ -k + -ATPase, CCO, LDH activity and ATP content in the mitochondria of rat liver were detected. The changes of ultrastructure of liver mitochondria were observed under transmission electron microscope. Results compared with the control group, the activity of SDH, Na+ -k + -ATPase, CCO, LDH and ATP in the model group decreased significantly (P < 0.01). Compared with the model group, the activity of these enzymes and the content of ATP in zuogui pill group were significantly increased (P<0.05 or P<0.01), and the pathological changes of the ultrastructure of liver mitochondria were significantly improved. Conclusion interfering with the energy metabolism barrier of liver mitochondria may be the mechanism of treating kidney-yin deficiency syndrome with "Yang zhong Yin seeking" of zuogui pill, which further reflects the compatibility significance of "Yang zhong Yin seeking" of zuogui pill.
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