黄芪发酵产物对小鼠急性肺损伤的近红外荧光活体成像研究

刘必旺; 赵换; 周文静; 路荣荣; 曹越

文章摘要

刘必旺,赵换,周文静,路荣荣,曹越.黄芪发酵产物对小鼠急性肺损伤的近红外荧光活体成像研究[J].中医药信息,2023,40(2):1-6

黄芪发酵产物对小鼠急性肺损伤的近红外荧光活体成像研究

Rearch of Lipopolysaccharide-induced ALI in Vivo by Astragalus Fermentation Products

投稿时间：2022-07-15 录用日期：2022-08-02

DOI：

中文关键词: 黄芪黄芪发酵产物脂多糖小鼠急性肺损伤近红外荧光活体成像

英文关键词: Astragalus membranaceus Astragalus fermentation products Lipopolysaccharide Acute lung injury in mice Near infrared fluorescence in vivo imaging

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作者	单位
刘必旺,赵换,周文静,路荣荣,曹越

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中文摘要:

目的:研究黄芪发酵产物(HF)拮抗脂多糖诱导的小鼠急性肺损伤(ALI)的干预效果,初步探讨其作用机制,并为其开发利用提供参考依据。方法:60只BALB/c小鼠按体质量随机分成空白对照组、模型组、舒普深组(0.3 g/kg)及HF低、中、高剂量组(12.5、25、50 g/kg),每组10只,根据文献方法采用吸入脂多糖法复制小鼠ALI模型,各组处理4周后,采用近红外荧光系统对小鼠活体成像进行观察,ELISA法检测小鼠血清TNF-α、IL-6、IL-1水平,Western blot法检测肺组织TLR4的表达,并进行肺部病理学观察。结果:模型组近红外荧光活体成像荧光信号最强,各给药组呈现不同程度的减弱的荧光信号。与模型组比较,舒普深组、HF中剂量组、HF高剂量组小鼠体质量不同程度的增加(P <0.05,P <0.01);HF中剂量组的TNF-α、IL-6的水平降低(P <0.05);HF高剂量组的TNF-α、IL-1、IL-6的水平降低(P <0.01,P <0.05);HF高剂量组TLR4表达水平降低(P <0.05)。病理学研究表明,HF能够降低脂多糖诱导的ALI小鼠的肺组织损伤程度。结论:黄芪发酵产物对脂多糖诱导的小鼠急性肺损伤具有保护作用,机制可能与其抑制TLR4通路发挥抗炎作用有关。

英文摘要:

Objective To study the effects of Astragalus fermentative product (HF) on lipopolysaccharide-induced acute lung injury in mice, and to explore its mechanism. Methods Sixty BALB/C mice were randomly divided into three groups according to Body Weight: Control Group, Model Group, positive drug group (Cefoperazone Sodium and Sulbactam Sodium for Injection, 0.3 g﹒kg-1) and HF low, middle and High Dose Group (12.5,25, 50 g﹒kg-1) , 10 mice in each group, according to the literature, Ali mice were induced by inhalation of lipopolysaccharide. After 4 weeks in each group, the in Vivo imaging of mice was observed by near-infrared fluorescence system, and the serum levels of TNF-a, IL-6 and IL-1 were detected by Elisa, western Blot was used to detect the expression of TLR4 in lung tissue. Results The fluorescence signal of near-infrared fluorescence in Vivo imaging was the strongest in the model group. Compared with the model group, the levels of TNF-a and IL-6 in the Middle Dose Group were significantly lower (p< 0.05) , and the levels of TNF-a, IL-1 and IL-6 in the high dose group were significantly lower (p< 0.05 or p< 0.01) . The expression of TLR4 IN HF High Dose Group was significantly lower than that in HF high dose group (p< 0.05). Conclusion The fermentation product of Astragalus membranaceus can improve the decrease of body weight, lower the level of serum inflammatory factors induced by Lipopolysaccharide, and lower the expression level of TLR4, the results showed that the fermentation product of Astragalus membranaceus had a good protective effect on ALI and protected lung tissue from the damage of inflammatory factors, which provided experimental basis for the development of new drugs and clinical application.

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