| 刘梅清；冯晓莉；刘媛媛；徐镱文；魏舒畅；刘晓霞；万玲娟.不同土质和生长年限甘草HPLC指纹图谱及3种成分含量测定研究[J].中医药信息,2023,40(7):47-52 |
| 不同土质和生长年限甘草HPLC指纹图谱及3种成分含量测定研究 |
| Study on Quality of Glycyrrhizae Radix et Rhizoma with Different Soil Quality and Growth Years |
| 投稿时间：2022-10-12 录用日期：2022-11-15 |
| DOI：10.19656/j.cnki.1002-2406.20230707 |
| 中文关键词: 不同土质 生长年限 甘草 含量测定 化学计量学 质量评价 |
| 英文关键词: Different soil quality Length of growth Glycyrrhizae Radix et Rhizoma Content determination Chemometrics Quality evaluation |
| 基金项目:甘肃省教育厅“双一流”科研重点项目 |
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| 中文摘要: |
| 目的：探究不同土质和生长年限甘草中甘草酸、甘草苷和甘草总黄酮的含量差异，并结合化学计量学分析方法为甘草质评质控体系的构建及其种植土质和适宜采收时间提供实验依据和参考。方法：采用HPLC法测定甘草苷和甘草酸含量，色谱条件：色谱柱为Symmetry C18色谱柱(4.6 mm×250 mm,5µm)，流速1.0 mL/min，检测波长237 nm，柱温26℃，流动相为乙腈-0.05%磷酸水梯度洗脱系统；采用紫外分光光度法测定甘草总黄酮含量，运用中药色谱指纹图谱相似度评价系统(2012.1版)对HPLC指纹图谱进行共有峰确定和相似度评价；运用OriginPro软件(2021版)对含量测定结果进行3D绘图分析；运用SPSS Statistics25统计学分析软件对样品进行聚类分析。结果：不同土质和生长年限栽培甘草HPLC指纹图谱共匹配24个共有峰成分，样品间相似度为0.917～0.991;3D绘图分析结果表明，不同土质和生长年限栽培甘草质量排序为3年沙地>5年沙地>3年土地>4年沙地>5年土地>4年土地，沙地种植的甘草质量明显高于土地；聚类分析结果可将不同土质和生长年限栽培甘草样品完全区分。结论：本研究所建立的甘草HPLC指纹图谱并结合化学计量学分析方法稳定可靠，可用于甘草质量评价研究与开发潜在标志物的探索。 |
| 英文摘要: |
| Objective:To investigate the content changes of glycyrrhizic acid,glycyrrhizin and total flavonoids in Glycyrrhizae Radix et Rhizoma with different soil quality and growing years,and to provide experimental basis and reference for the construction of quality control system of Glycyrrhizae Radix et Rhizoma,planting soil quality and suitable harvesting time.Methods:HPLC was used to determine glycyrrhizin and glycyrrhizic acid.The chromatographic conditions were as follows,Symmetry C18 column was(4.6 mm × 250 mm,5 µm),flow rate was 1.0 mL/min,detection wavelength was 237 nm,column temperature was 26 ℃,mobile phase was Acetonitrile-0.05% phosphoric acid gradient elution system.The total flavonoids of Glycyrrhizae Radix et Rhizoma were determined by ultraviolet spectrophotometry.The TCM chromatographic fingerprint similarity evaluation system(2012 Version Ⅰ) was used to determine the common peak and evaluate the similarity of HPLC fingerprint.The content determination results were analyzed by 3D mapping using OriginPro software(2021 edition).SPSS Statistics 25 was used for cluster analysis.Results:The HPLC fingerprints of cultivated Glycyrrhizae Radix et Rhizoma with different soil quality and growth years matched 24 common peak components,and the similarity between samples was 0.917-0.991.The results of 3D mapping analysis showed that the quality of cultivated Glycyrrhizae Radix et Rhizoma in different soil quality and growth years was ranked as 3-year sandy land,5-year sandy land,3-year land,4-year sandy land,5-year land and 4-year land in order,and the quality of cultivated Glycyrrhizae Radix et Rhizoma in sandy land was significantly higher than that in land.The results of cluster analysis could completely distinguish the cultivated Glycyrrhizae Radix et Rhizoma samples with different soil quality and growth years.Conclusion:The HPLC fingerprint of Glycyrrhizae Radix et Rhizoma combined with the chemometrics analysis method is stable and reliable,which can be applied to the quality evaluation of Glycyrrhizae Radix et Rhizoma and the exploration of potential markers. |
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