| 聂安政；边猛；朱春胜.不同剂量细辛水煎液对大鼠肝、肾、肺毒性的影响[J].中医药信息,2023,40(7):53-57 |
| 不同剂量细辛水煎液对大鼠肝、肾、肺毒性的影响 |
| Effects of Different Doses of Asarum Decoction on Liver,Kidney and Lung Toxicity in Rats |
| 投稿时间：2022-11-18 录用日期：2022-12-06 |
| DOI：10.19656/j.cnki.1002-2406.20230708 |
| 中文关键词: 细辛 肝毒性 肾毒性 肺毒性 |
| 英文关键词: Asarum Hepatotoxicity Renal toxicity Pulmonary toxicity |
| 基金项目:河南省医学科技攻关计划联合共建项目(LHGJ20190274)；中华医学会临床药学分会吴阶平基金会专项科研基金课题(LCYX-Q025) |
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| 中文摘要: |
| 目的：观察不同剂量的细辛水煎液对SD大鼠肝、肾、肺组织形态及肝、肾功能的影响，探讨细辛的肝、肾、肺毒性。方法：50只SPF级雄性SD大鼠随机分为正常组、低剂量组、中剂量组、极高剂量组，每组各10只，正常组灌胃生理盐水，其余各组分别灌胃给予3、6、12、24 g/kg细辛水煎液，连续28 d。分别于实验第7、14、21、28天尾静脉取血，检测血清ALT、AST、CRE、BUN水平，第28天处死动物，取肝、肾、肺组织采用HE染色法观察组织形态。结果：中剂量组(6 g/kg)大鼠肺泡壁轻度增厚，伴少量炎性细胞浸润；高剂量组(12 g/kg)大鼠第14、28天血清ALT水平显著升高(P<0.05)，第28天血清AST水平显著升高(P<0.05)，肝组织细胞颗粒变性，局部血管周围可见淋巴细胞灶性浸润，肾组织少量炎性细胞浸润，肺组织肺泡壁中度增厚，大量淋巴细胞炎性浸润；极高剂量组(24 g/kg)大鼠第14、21天血清ALT水平显著升高(P<0.01,P<0.05)，第14、21、28天血清ALT水平显著升高(P<0.05,P<0.01)，第7、21天血清BUN水平显著升高(P<0.05)，肝组织细胞颗粒变性，胞质疏松淡染呈颗粒状，局部血管周围淋巴细胞灶性浸润，肾组织少量炎性细胞浸润伴中等量的集合管扩张，肺组织肺泡壁增厚，大量淋巴细胞炎性浸润，局部支气管内上皮细胞脱落。结论：6～24 g/kg细辛水煎液可诱导不同程度的肺损伤，12～24 g/kg细辛水煎液可诱导不同程度的肝、肾损伤。 |
| 英文摘要: |
| Objective:To observe the effects of different doses of asarum decoction on the morphology and functions of liver,kidney and lung in SD rats,and to investigate the toxicity of asarum to liver,kidney and lung.Methods:50 SPF SD rats were randomly divided into the normal group,the low-dose group,the medium-dose group,the high-dose group and the extremely high-dose group,with 10 rats in each group.The normal group was given normal saline,the rest groups were given different doses of asarum decoction(3 g/kg,6 g/kg,12 g/kg and 24 g/kg) by intragastric administration for 28 days.Blood was collected from the tail vein on day 7,day 14,day 21 and day 28 to detect the serum levels of ALT,AST,CRE and BUN.The tissue samples of liver,kidney and lung were collected and stained with HE to observe the tissue morphology.Results:The alveolar wall was slightly thickened in the medium-dose group with a small amount of inflammatory cell infiltration.The serum level of ALT was significantly increased on day 14 and day 28(P<0.05);the serum level of AST was significantly increased on day 28(P<0.05),there were hepatocyte granular degeneration,focal lymphocyte infiltration around local blood vessels,a small amount of inflammatory cell infiltration in renal tissue,moderate thickening of alveolar wall in lung tissues and a large number of lymphocyte inflammatory infiltrates in the high-dose group.In the extremely high-dose group,the serum level of ALT was significantly increased on day 14 and day 21(P<0.05),the serum level of ALT was significantly increased on day 14,day 21 and day 28(P<0.05,P<0.01),and the serum level of BUN was significantly increased on day 7 and day 21(P<0.05);there were granular degeneration of liver cells,loose and light stained granular cytoplasm,focal infiltration of local perivascular lymphocytes,a small amount of inflammatory cell infiltration in renal tissues with a moderate amount of collecting duct dilatation,thickening of alveolar wall in lung tissues,a large number of inflammatory infiltration of lymphocytes,and local epithelial cell shedding in bronchus.Conclusion:6-24 g/kg asarum water decoction can induce different degrees of lung injury,12-24 g/kg asarum water decoction can induce different degrees of liver and kidney injuries. |
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