| 张晓川;娄素卉;聂安政;李晓萍.基于蛋白质组学技术探讨榆栀止血颗粒治疗异常子宫出血大鼠的作用机制[J].中医药信息,2023,40(8):9-14 |
| 基于蛋白质组学技术探讨榆栀止血颗粒治疗异常子宫出血大鼠的作用机制 |
| Action Mechanism of Yuzhi Zhixue Granule in Treating Rats with AUB Based on Proteomics Technology |
| 投稿时间：2023-01-17 录用日期：2023-02-01 |
| DOI：10.19656/j.cnki.1002-2406.20230802 |
| 中文关键词: 榆栀止血颗粒 蛋白质组学 异常子宫出血 平行反应监测 |
| 英文关键词: Yuzhi Zhixue Granule Proteomics Abnormal uterine bleeding Parallel reaction monitoring |
| 基金项目:河南省医学科技攻关计划项目(LHGJ20190224)；郑州大学第一附属医院横向课题(K2020-0003) |
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| 中文摘要: |
| 目的：使用蛋白质组学技术研究榆栀止血颗粒对异常子宫出血大鼠蛋白表达的影响，探究其作用机制。方法：除对照组外通过药物致早孕大鼠流产的方法建立实验模型，30只SPF级Wistar妊娠雌鼠随机分为模型组、给药组和对照组，每组10只。给药组连续给予榆栀止血颗粒7 d，于第1、2、3、7天检测各组大鼠出血量。采用定量蛋白质组学分析榆栀止血颗粒对大鼠血清中蛋白表达差异的影响，并分析差异蛋白参与的信号通路。针对筛选出的差异蛋白和信号通路，利用平行反应监测技术进行验证。结果：模型组大鼠从第1天开始子宫出血量较对照组显著增加（P <0.01）；与模型组比较，给药组大鼠从第1天开始子宫出血量显著减少（P <0.01）。共定量得到5 496个蛋白质。给药组与模型组之间有84个差异蛋白（上调28个，下调56个）。差异蛋白主要富集于PI3K/Akt信号通路、补体和凝血级联、血小板激活等信号通路。选择玻连蛋白、纤溶酶原、丝氨酸蛋白酶抑制剂D1素辅因子Ⅱ等进行PRM验证，验证结果与TMT结果一致。结论：榆栀止血颗粒能够通过调节玻连蛋白、纤溶酶原等蛋白的表达水平，实现对异常子宫出血大鼠的治疗作用。 |
| 英文摘要: |
| Objective: To study the effect of Yuzhi Zhixue Granule(YZZXG) on protein expressions in rats with abnormal uterine bleeding(AUB), and to explore its mechanism in treating AUB. Methods: 30 SPF Wistar female pregnant rats were randomly divided into the control group, the model group and the medication group, with 10 rats in each group. The model drug-induced abortion in early pregnancy were established in the rats of the model group and the medication group. The medication group was administrated with YZZXG for 7 days, the amount of bleeding in each group was measured on day 1, day 2, day 3 and day 7. Quantitative proteomics was used to analyze the effect of YZZXG on the differential expression of serum proteins in the rats and to analyze the signaling pathway of differential proteins. And the differential proteins and signaling pathways were verified by parallel reaction monitoring. Results: The amount of uterine bleeding was significantly increased in the model group from day 1(P < 0. 01); compared with that in the model group, the amount of uterine bleeding was significantly reduced in the medication group from day 1(P < 0. 01). The results showed that 5,496 proteins were obtained by total quantitation. There were 84 differential proteins between the medication group and the model group; among them, 28 were up-regulated and 56 were downregulated. Differential proteins were mainly enriched in PI3K/Akt signaling pathway, complement and coagulation cascade, platelet activation and other signaling pathways. Vitronectin, plasminogen and serine protease inhibitor D1 heparin cofactor Ⅱ were selected for PRM verification, and the verification results were consistent with the TMT results. Conclusion: YZZXG can regulate the protein expressions of hyalenin and plasminogen to achieve the therapeutic effect in treatment of AUB in rats. |
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