| 赵灵灵;周政;贾文瑞;王娟.基于代谢组学探讨金藤清痹颗粒治疗类风湿关节炎的作用机制[J].中医药信息,2023,40(9):16-22 |
| 基于代谢组学探讨金藤清痹颗粒治疗类风湿关节炎的作用机制 |
| Action Mechanism of Jinteng Qingbi Granule in Treatment of RA Based on Metabonomics |
| 投稿时间：2023-02-02 录用日期：2023-02-27 |
| DOI：10.19656/j.cnki.1002-2406.20230903 |
| 中文关键词: 金藤清痹颗粒 类风湿关节炎 代谢组学 差异代谢物 |
| 英文关键词: Jinteng Qingbi Granule Rheumatoid arthritis Metabonomics Differential metabolites |
| 基金项目:河南省医学科技攻关计划联合共建项目(LHGJ20190275)；郑州大学第一附属医院横向课题。 |
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| 中文摘要: |
| 目的：基于代谢组学的方法探讨金藤清痹颗粒治疗类风湿关节炎（RA）的作用机制。方法：将SPF级健康SD雄性大鼠30只随机分为对照组（Control组）、模型组（Model组）、双氯芬酸钠阳性药组（SLFSN组）、金藤清痹颗粒高剂量组（JTQB-H组）和金藤清痹颗粒低剂量组（JTQB-L组）。Control组和Model组大鼠灌胃给予生理盐水，其余各组灌胃给予相应药物，给药28 d。除对照组外，其余各组采用胶原诱导RA大鼠模型。HE染色观察大鼠踝关节病理变化，酶标仪检测大鼠血清相关炎症指标，UPLC-Q-TOF/MS筛选金藤清痹颗粒干预RA大鼠后的差异代谢物。结果：与Control组比较，Model组大鼠踝关节肿胀明显，滑膜细胞增生并伴随大量炎性细胞浸润，显著升高血清TNF-α、IL-1β、RF水平（P <0.01）；与Model组比较，金藤清痹颗粒各剂量组可明显缓解RA模型大鼠关节肿胀度，改善踝关节炎性浸润，JTQB-H组可显著降低RA大鼠血清TNF-α、IL-1β水平（P <0.05）；代谢组学共筛选出包括DL-丝氨酸、L-赖氨酸、高脯氨酸在内的12个差异代谢物；KEGG通路分析显示，金藤清痹颗粒治疗RA主要通过调控甘氨酸，丝氨酸和苏氨酸代谢、亚油酸代谢、苯丙氨酸代谢等通路来发挥疗效。结论：金藤清痹颗粒治疗RA的作用机制可能与调控多条氨基酸代谢通路有关。 |
| 英文摘要: |
| Objective: To investigate the mechanism of Jinteng Qingbi Granule in treatment of rheumatoid arthritis(RA) based on metabolomics. Methods: 30 SD rats were randomly divided into the control group, the model group, the Diclofenac sodium group, the Jinteng Qingbi(JTQB) groups of high-dose and low-dose.RA model was induced by using collagen. After 28 days of intervention, pathological changes in the ankle joint area of rats were observed by HE staining, serum-related inflammatory indexes were detected by Elisa method, and differential metabolites of JTQB were screened out by UPLC-Q-TOF/MS. Results: Compared with those in the control group, the ankle swelling, synoviocyte proliferation and inflammatory cell infiltration could be seen, the serum levels of TNF-α, IL-1β and RF were significantly increased in the model group(P < 0. 01). Compared with those in the model group, the joint swelling and inflammatory infiltration were significantly alleviated, and the serum levels of TNF-α and IL-1β were significantly reduced in the JTQB-H group( P < 0. 05). Metabolomics screened 12 different metabolites including DL-serine, L-lysine and hyperproline. KEGG analysis showed that the treatment of RA with JTQB Granule was mainly through the regulation of metabolisms of glycine, serine, threonine, linoleic acid and phenylalanine. Conclusion: The action mechanism of Granule JTQB may be related to the regulation of multiple amino acid metabolic pathways in treatment of RA. |
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