| 周佳俞；陈丽；康瀚云；丁雅容；王巍；袁忠行；雷嘉欣；周忠志.糖尿病阴证大鼠创面模型的制备[J].中医药信息,2023,40(9):46-53 |
| 糖尿病阴证大鼠创面模型的制备 |
| Establishment of Wound Model of Diabetes Rats with Yin Syndrome |
| 投稿时间：2023-03-21 录用日期：2023-04-10 |
| DOI：10.19656/j.cnki.1002-2406.20230908 |
| 中文关键词: 糖尿病阴证 链脲佐菌素 氢化可的松 大鼠模型 |
| 英文关键词: Diabetes with yin syndrome Streptozotocin Hydrocortisone Rat model |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的：探讨糖尿病阴证大鼠创面模型的制备方法。方法：雄性SD大鼠36只，随机分为正常创面组、糖尿病创面组和糖尿病阴证创面组。除正常创面组外，其余两组大鼠采用2 mL高脂乳剂灌胃14 d，予以一次性腹腔注射50 mg/kg STZ-柠檬酸钠混合液，糖尿病阴证创面组大鼠按2 mg/（kg·d）肌内注射氢化可的松；同时正常创面组灌胃饮用水，腹腔注射生理盐水。7 d后3组大鼠均进行创面手术，观察大鼠一般情况，进行OGTT试验，观察胰腺组织形态以及创面愈合率。结果：糖尿病阴证创面组造模成功率91.7%；与正常创面组比较，糖尿病创面组与糖尿病阴证创面组大鼠血糖明显升高（P <0.05），且存在胰岛素抵抗；脏器指数显示，糖尿病阴证创面组较其余两组降低（P <0.05）；胰腺HE染色表明，正常创面组大鼠胰腺组织肥厚，胰岛完整，而糖尿病创面组与糖尿病阴证创面组胰岛边界模糊，β细胞消融。创面形态方面，糖尿病阴证创面组相较于正常创面组和糖尿病创面组的愈合率较低（P <0.01）,HE染色可见糖尿病阴证创面组全层皮肤较前两组薄，表皮生长不平整，炎细胞浸润，细胞排列紊乱。结论：给予SD大鼠2 mL高脂乳剂灌胃14 d后，予以一次性腹腔注射50 mg/kg STZ-柠檬酸钠混合液联合2 mL/kg肌内注射氢化可的松注射液7 d后行创面手术，可建立稳定高效的糖尿病阴证大鼠创面模型。 |
| 英文摘要: |
| Objective: To explore the preparation method of wound model of diabetes rats with yin syndrome.Methods: 36 Sprague Dawley male rats were randomly divided into three groups: normal wound group(group A), group of diabetes and wound(group B), and group of diabetes and wound with yin syndrome(group C).2 mL high-fat emulsion was administered by gavage for 14 days, 50 mg/kg STZ sodium citrate mixture was intraperitoneally injected once, and 2 mg/kg/d hydrocortisone was given by intramuscular injection. After 7 days, wound was made surgically. General condition of the animals, OGTT test, pancreatic tissue morphology, and wound healing rate were recorded. Results: The success rate of modeling for group C was 91. 7%. Compared with group A, the blood glucose of group B and group C was significantly increased(P <0. 05), and there was insulin resistance in these 2 groups. Group C gave lower organ index than the other two groups(P < 0. 05). HE staining of pancreatic tissue showed that the pancreatic tissue of rats in the group A was hypertrophic and the islets were intact, while the boundary of the islets in the group B and group C was blurred and the β cells were ablated. The wound healing rate was lower in group C than that in group A and group B(P < 0. 01), and HE staining showed that the full-layer skin at the wound in group C was thinner than that in the group A and B. The epidermal growth was uneven, there were inflammatory cells infiltrated, and the cell arrangement was in disorder in group C. Conclusion: Based on this, we can establish a stable wound model of diabetes rats with yin syndrome. |
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