| 闫叶星;赵崧;沈洪.健脾补肾、清肠化湿方对溃疡性结肠炎模型大鼠炎症及肠道菌群的影响[J].中医药信息,2023,40(8):1-8 |
| 健脾补肾、清肠化湿方对溃疡性结肠炎模型大鼠炎症及肠道菌群的影响 |
| Effect of Jianpi Bushen Qingchang Huashi Formula on Inflammation and Intestinal Flora of SD Rats of Ulcerative Colitis Model |
| 投稿时间：2023-03-27 录用日期：2023-04-10 |
| DOI：10.19656/j.cnki.1002-2406.20230801 |
| 中文关键词: 健脾补肾 清肠化湿方 溃疡性结肠炎 肠道菌群 |
| 英文关键词: Jianpi Bushen Qingchang Huashi Formula Ulcerative colitis Intestinal flora |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的：基于微生物组学方法对溃疡性结肠炎模型大鼠粪便菌群进行研究，探讨健脾补肾、清肠化湿方对溃疡性结肠炎模型大鼠肠道菌群的影响。方法：66只健康SPF级SD大鼠依据随机数字表随机分为空白对照组，模型组，健脾补肾、清肠化湿方组，补肾组，健脾组和清肠组等6组。各给药组依照成人用药浓度灌胃治疗，隔日称量大鼠体质量，10 d后处死大鼠并制作病理切片，计算各组病理评分；酶联免疫吸附法检测血清IL-1β、TNF-α和IL-10水平；基于16S rRNA扩增与高通量测序技术检测大鼠粪便菌群构成及丰度。结果：健脾补肾、清肠化湿方及其拆方明显改善了溃疡性结肠炎组织的病理评分，缓解了结肠黏膜的组织病理学损伤，减少了黏膜杯状细胞的破坏，显著降低了血清IL-1β和TNF-α水平，提高了血清IL-10的水平；通过16S rRNA序列分析验证，发现健脾补肾、清肠化湿方及其拆方有效改善了SD大鼠肠道菌群失调的情况。结论：健脾补肾、清肠化湿方可改善溃疡性结肠炎模型大鼠的肠道炎症反应和组织病理学损伤，并对溃疡性结肠炎模型大鼠的肠道菌群有显著的调节作用。 |
| 英文摘要: |
| Objective: To study the fecal microbiota of ulcerative colitis model rats based on microbiomics, and explore the effects of Jianpi Bushen Qingchang Huashi(JBQH)Formula on the intestinal microbiota of the same rats. Methods: 66 healthy SPF-grade SD rats were randomly divided into blank control group, model group, JBQH Formula group, kidney-supplementing group, spleen-fortifying group and intestine-clearing group. Each medication group was treated by gavage according to the adult drug concentration, and the body weight of the rats was measured every other day. After 10 days, the rats were euthanized and pathological sections were made, and the pathological scores of each group were calculated. Serum levels of IL-1β, TNF-α and IL-10 were measured by enzyme-linked immunosorbent assay(ELISA), and the composition and abundance of rat fecal flora were detected by 16S rRNA amplification and high-throughput sequencing technology. Results: JBQH Formula, and its disassembled formulae significantly improved the pathological score of ulcerative colitis, alleviated the histopathology damage of colonic mucosa, reduced the damage of mucosal goblet cell, significantly reduced serum levels of IL-1 β and TNF-α, and increased serum IL-10 level. Through 16S r RNA sequence analysis verification, it was found that JBQH Formula, and its disassembled formulae effectively improved the imbalance of gut microbiota in SD rats. Conclusion: JBQH Formula can improve the intestinal inflammatory reaction and histopathological damage in ulcerative colitis model rats, and has a significant regulatory effect on the intestinal flora of ulcerative colitis model rats. |
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