| 甘安娜；王诗宇；晋炎君；吴博；颜廷旭；贾英.益智醇提物抗抑郁活性研究[J].中医药信息,2024,41(2):1-8 |
| 益智醇提物抗抑郁活性研究 |
| Antidepressant Activity of Sharpleaf Glangal Fruit Alcohol Extract |
| 投稿时间：2023-06-19 录用日期：2023-07-28 |
| DOI：10.19656/j.cnki.1002-2406.20240201 |
| 中文关键词: 益智 抗炎 抗氧化 抑郁症 行为学 |
| 英文关键词: Sharpleaf glangal fruit Anti－inflammation Antioxidation Depression Ethology |
| 基金项目: |
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| 中文摘要: |
| 目的：研究益智醇提物（AOY）的抗抑郁活性，初步明确其作用机制。方法：采用小鼠耳肿胀实验以及小鼠棉球肉芽肿实验考察AOY的抗炎能力；采用DPPH自由基、ABTS自由基、羟基自由基以及超氧阴离子自由基清除力的体外抗氧化模型考察AOY的抗氧化能力；采用慢性不可预知温和应激（CUMS）小鼠模型，用强迫游泳实验（FST）、糖偏好实验（SPT）以及悬尾实验（TST）等行为学实验考察其抗抑郁活性；通过测定小鼠海马中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH－Px）、一氧化氮合酶（iNOS）、环氧合酶－2（COX－2）的水平和核转录因子（NF－κB）、炎症小体（NLRP3）以及白介素－1β（IL－1β）的含量，探究其作用机制。结果：益智醇提物能够抑制小鼠的急性炎症和慢性炎症；且在一定浓度下与维生素C（VC）的抗氧化水平相当，表明AOY有很好的抗氧化活性；AOY能显著增强糖偏好实验中小鼠的糖偏好程度，并显著降低小鼠在悬尾实验以及强迫游泳实验中的不动时间。给予AOY后，小鼠海马体中SOD和GSH－Px活性升高，iNOS、COX－2、NF－κB、NLRP3和IL－1β含量降低。结论：AOY具有良好的抗炎抗氧化活性，且AOY能同时作用于炎症反应和氧化应激通路发挥抗抑郁作用。 |
| 英文摘要: |
| Objective: To study the antidepressive activity of sharpleaf glangal fruit,Alpiniae Oxyphyllae Fructus alcohol extract (AOY), and preliminary clarify its mechanism of action. Methods: Mouse ear swelling test and mouse cotton ball granuloma test were used to investigate the anti－inflammatory ability of AOY; the antioxidant capacity of AOY was investigated by using the antioxidant model of DPPH free radical, ABTS free radical, hydroxyl free radical and superoxide anion free radical scavenging power in vitro; a mouse model of chronic unpredictable mild stress (CUMS) was used to investigate its antidepressant activity by forced swimming test (FST), sugar preference test (SPT) and tail suspension test (TST). The action mechanism of AOY was explored by measuring the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH－Px), nitric oxide synthase (iNOS), cyclooxygenase－2(COX－2), nuclear transcription factor (NF－κB), inflammatory body (NLRP3) and interleukin－1β(IL－1β) in the hippocampus of mice. Results: Alcohol extract of AOY inhibits acute and chronic inflammation in mice. AOY was comparable to the antioxidant level of vitamin C (VC) at a certain concentration, indicating that it has good antioxidant activity. AOY could significantly enhance the sugar preference degree of mice in the sugar preference experiment, and significantly reduce the immobile time of mice in the tail suspension experiment and the forced swimming experiment. After AOY administration, SOD and GSH－Px activities increased in the hippocampus of mice, while iNOS, COX－2,NF－κB, NLRP3 and IL－1β contents decreased. Conclusion: Sharpleaf glangal fruit has good anti－inflammatory and antioxidant activity, and AOY can act on both inflammatory response and oxidative stress pathways to play an antidepressant role. |
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