| 刘阳阳；张云云；李洁；丁永芳；彭蕴茹.瓜蒌薤白汤正丁醇部位对Ⅱ型心肾综合征大鼠内质网应激的调控作用[J].中医药信息,2024,41(2):26-32 |
| 瓜蒌薤白汤正丁醇部位对Ⅱ型心肾综合征大鼠内质网应激的调控作用 |
| Regulation of N－Butanol Fraction of Gualou Xiebai Decoction on Endoplasmic Reticulum Stress in Rats with Type II Cardiorenal Syndrome |
| 投稿时间：2023-07-11 录用日期：2023-08-25 |
| DOI：10.19656/j.cnki.1002-2406.20240205 |
| 中文关键词: 瓜蒌薤白汤 II型心肾综合征 内质网应激 |
| 英文关键词: Gualou Xiebai Decoction Type II cardiorenal syndrome Endoplasmic reticulum stress |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的：观察瓜蒌薤白汤（Gualou Xiebai Decoction，GXD）正丁醇部位对II型心肾综合征大鼠内质网应激的影响，探讨瓜蒌薤白汤对心脏和肾脏的保护作用。方法：32只SPF级健康雄性Wistar大鼠随机分为假手术组、模型组和瓜蒌薤白汤高、低剂量组。除假手术组外，其余各组采用结扎左冠状动脉前降支的方法复制II型心肾综合征大鼠模型，灌胃给予相应药物10周后，ELISA法检测血清中NT－proBNP、Cys－C的含量；取心脏、肾脏组织，HE染色观察心脏、肾脏的细胞形态学变化，Masson染色检测组织胶原沉积和纤维化程度；透射电镜观察内质网超微结构；RT－PCR检测心、肾组织I、III型胶原含量；Western blot检测内质网相关蛋白ATF－6、GRP78、XBP－1s、ATF－4、p－eIF2α、CHOP的表达。结果：与假手术组比较，模型组大鼠血清中NT－proBNP、Cys－C的水平明显升高（P < 0.01），瓜蒌薤白汤高、低剂量组较模型组明显降低（P < 0.01）。与模型组比较，瓜蒌薤白汤组能显著改善由慢性心衰引起的大鼠心脏、肾脏组织病理学改变；透射电镜观察发现，模型组大鼠内质网肿胀、断裂，核糖体脱落，瓜蒌薤白汤组心脏、肾脏内质网基本恢复正常。RT－PCR结果表明，模型组心脏、肾脏中I、III型胶原mRNA表达明显高于假手术组，瓜蒌薤白汤高、低剂量组明显低于模型组（P < 0.01）；瓜蒌薤白汤组内质网相关蛋白ATF－6、GRP78－、XBP－1s、ATF－4、p－eIF2α、CHOP的表达显著低于模型组（P < 0.01）。结论：瓜蒌薤白汤对II型心肾综合征大鼠心脏、肾脏具有明显的保护作用，且该作用可能与抑制内质网应激有关。 |
| 英文摘要: |
| Objective：To observe the effect of Gualou Xiebai Decoction （GXD） n - butanol fraction on endoplasmic reticulum stress in rats with type II cardiorenal syndrome， and explore the protective effects of GXD on the heart and kidneys. Methods： Thirty - two healthy male Wistar rats with SPF grade were randomly divided into sham operation group， model group， GXD high - dose and low - dose groups. Except the sham operation group， the other groups were treated with ligation of the anterior descending branch of the left coronary artery to produce the rat model of type II cardio - renal syndrome. The contents of NT - proBNP and Cys - C in serum were detected by ELISA after 10 weeks of administration of the corresponding drugs. Heart and kidney tissues were taken， and cellular morphological changes in the heart and kidneys were observed with HE staining，and collagen deposition and fibrosis degree were detected with Masson staining. The ultrastructure of the endoplasmic reticulum was observed by transmission electron microscopy. RT - PCR was used to determine the levels of collagen type I and III in the heart and kidney tissue. The endoplasmic reticulum related proteins ATF - 6， GRP78， XBP - 1s， ATF - 4， p - eIF2α and CHOP were detected by Western blot. Results： Compared with the sham surgery group， the levels of NT - proBNP and Cys - C in the serum of rats in the model group significantly increased （P < 0.01）， and the levels of NT - proBNP and Cys - C in the groups of GXD were significantly lower than those in the model group （P < 0.01）. Compared with the model group， the GXD groups could significantly improve the pathology in the heart and kidney tissues of rats with chronic heart failure. The transmission electron microscopy showed that the endoplasmic reticulum of the model group rats was swollen，broken， and ribosome fell off，and the endoplasmic reticulum of the GXD group basically returned to normal.The RT - PCR results showed that the mRNA expression of type I and III collagen in the heart and kidney of the model group was significantly higher than that of the sham group， while that of GXD high - dose and low - dose groups was significantly lower than that of the model group （P < 0.01）. The expressions of the endoplasmic reticulum - related proteins ATF - 6， GRP78， XBP - 1s， ATF - 4， p - eIF2α and CHOP were significantly lower in the GXD group than those in the model group. Conclusion： Gualou Xiebai Decoction has obvious protective effects on the heart and kidney of type II cardiorenal syndrome rats， and this effect may be related to the inhibition of endoplasmic reticulum stress. |
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