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| 应用基因芯片技术分析甘草酸改善大鼠酒精性肝损伤的作用机制 |
| Analysis of the improvement mechanism of glycyrrhizic acid on alcoholic liver injury rats by gene chip technology |
| 投稿时间：2021-01-30 录用日期：2021-03-16 |
| DOI： |
| 中文关键词: 酒精性肝损伤 甘草酸 可变剪切 基因芯片 |
| 英文关键词: |
| 基金项目:新疆医科大学创新(XYDCX5201515) |
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| 摘要点击次数: 59 |
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| 中文摘要: |
| 目的 运用基因芯片技术检测甘草酸对大鼠酒精性肝损伤保护作用的分子机制。方法 将45只大鼠随机分为3组：对照组、模型组、甘草酸组,连续灌服56度白酒,构建大鼠酒精性肝损伤模型。连续灌喂8周后,处理动物,测定ALT、AST水平；抽提肝脏组织的RNA,全基因组表达谱芯片检测,运用GO和KEGG Pathway富集分析。结果 与正常组比较,模型组血清AST和ALT含量显著升高(P<0.05),肝组织出现显著的肝损伤；与模型组相比,甘草酸组能显著改善肝组织病变,降低肝血清AST和ALT含量(P<0.05)。芯片结果分析显示,甘草酸组与模型组共筛选出7482个差异可变剪切RNA(Splicing Index<-3或>3,P<0.05),其中差异可变剪切的编码RNA有6512个,差异可变剪切的非编码RNA有970个；对差异可变剪切的编码RNA进行GO和Pathway富集分析,结果显示,主要与乙醇分解代谢、肝星状细胞活化、氧化还原酶活性、氨基酸合成代谢、脂肪酸的生物合成、Notch信号通路、酮体的合成与降解等功能或通路有关。结论 甘草酸在改善大鼠酒精性肝损伤病变过程中存在着大量差异可变剪切的RNA,提示甘草酸可通过调节多基因可变剪切改善酒精性肝损伤病变。 |
| 英文摘要: |
| Objective To investigate the protective mechanism of glycyrrhizic acid in alcoholic liver injury rats. Methods Randomly divided 45 rats into three groups: control group, model group and glycyrrhizic acid group. Using 56 degree Erguotou distillate spirit intragastric administrationSmethodSsetSup the model of alcoholic liver injury in rats. At the 8th week with continuous intragastric administration, Blood were collected for determination the level of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The total RNA was extracted from the hepatic tissue. Then, the variable of alternative splicing were detection and analysised with the whole genome expression array. Final, the function of differentially alternative splicing coding RNA through Gene Ontology and KEGG Pathway enrichment analysis. Results Compared with the normal group, the serum AST and ALT levels in the model group were significantly increased (P<0.05), and the liver tissue showed significant liver damage. Compared with the model group, glycyrrhizic acid group can significantly improve liver tissue lesions and reduce AST and ALT content in liver serum (P<0.05). The microarray analysis showed: A total of 7482 RNA (Splicing Index<-3 or >3, P<0.05) were screened, there were 6512 coded RNA for alternative splicing and 970 non-coding RNA between glycyrrhizic acid group and the model group. GO and KEGG Pathway enrichment were analysised of coded RNA, the result display, it were mainly related to ethanol catabolic process, hepatic stellate cell activation, oxidoreductase activity, amino acid anabolism, fatty acid biosynthesis, Notch signaling pathway, ketone synthesis and degradation and other functions or pathways. Conclusions There were lots of variable alternative splicing RNA in the progress of alcoholic liver injury rats protectioned with glycyrrhizic acid, suggested that glycyrrhizic acid can improve alcoholic liver injury by regulating lots of alternative splicing RNA. |
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