宣白承气汤对痰热腑实型哮喘大鼠肺组织病理损伤及Th17/Treg失衡的影响

王禹清；张露；郭松伟；李晨；陆柯；张庆祥

文章摘要

王禹清；张露；郭松伟；李晨；陆柯；张庆祥.宣白承气汤对痰热腑实型哮喘大鼠肺组织病理损伤及Th17/Treg失衡的影响[J].中医药信息,2024,41(2):33-38

宣白承气汤对痰热腑实型哮喘大鼠肺组织病理损伤及Th17/Treg失衡的影响

Effect of Xuanbai Chengqi Decoction on Pathological Damage and Th17/Treg Imbalance of Lung Tissue of Asthmatic Rats with Phlegm－Heat and Fu－Organ Excess

投稿时间：2023-04-22 录用日期：2023-06-01

DOI：10.19656/j.cnki.1002-2406.20240206

中文关键词: 宣白承气汤痰热腑实哮喘病理改变 Th17/Treg

英文关键词: Xuanbai Chengqi Decoction Phlegm－heat and fu－organ excess Asthma Pathological changes Th17/Treg

基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目）

作者	单位
王禹清；张露；郭松伟；李晨；陆柯；张庆祥

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中文摘要:

目的：观察宣白承气汤对痰热腑实型哮喘大鼠肺组织病理损伤情况及肺泡灌洗液（BALF）中Th17/Treg失衡的影响。方法：将50只Wistar雄性大鼠随机分为空白对照组（A组）、模型组（B组）、定喘汤组（C组）、宣白承气汤组（D组）和地塞米松组（E组），每组10只。利用OVA诱导哮喘模型，并施加痰热腑实因素，造模成功后，C组、D组和E组分别按1.5 mL/100 g给予定喘汤（0.067 g/mL）、宣白承气汤（0.036 g/mL）和地塞米松溶液［0.05 mg/（100 g·d）］，A组和B组按等比例给予生理盐水，1次/d，连续用药14 d后，观察各组大鼠一般情况；HE染色观察大鼠肺组织病理改变情况，酶联免疫吸附法（ELISA）比较大鼠肺泡灌洗液中IL－6、转化生长因子－β（TGF－β）、IL－17、IL－10的含量。结果：病理切片结果显示，A组未见炎性细胞浸润，肺泡扩张良好、支气管黏膜正常；B组可见大量嗜酸性粒细胞及淋巴细胞浸润等支气管哮喘病理表现；C组、D组和E组细胞炎性浸润均减少，病理情况均有所改善。ELISA结果显示，与A组比较，B组肺泡灌洗液中IL－6和IL－17水平显著上升（均P < 0.01）；与B组比较，C组、D组、E组IL－6和IL－17水平均下降（均P < 0.01）；与A组比较，B组肺泡灌洗液中TGF－β和IL－10水平均下降（均P < 0.01）；与B组比较，D组两种细胞因子水平均显著上升（均P < 0.01）；C组TGF－β水平上升（P < 0.05），IL－10水平明显上升（P < 0.01）；E组IL-10水平明显上升（P < 0.01），但TGF－β上升不明显（P > 0.05）。结论：宣白承气汤可以通过泄热通腑的方法治疗痰热腑实型哮喘，改善哮喘症状，缓解大鼠肺组织病理损伤，改变肺泡灌洗液中IL－6、TGF－β、IL－17、IL－10的含量，从而调节Th17/Treg失衡。

英文摘要:

Objective：To observe the effect of Xuanbai Chengqi Decoction （XCD） on pathological damage of lung tissue and imbalance of Th17/Treg in broncho－alveolar lavage fluid （BALF） in asthmatic rats with phlegm－heat and fu－organs excess. Methods： Fifty male Wistar rats were randomly divided into Group A （blank control group）， Group B （model group）， Group C （Dingchuan decoction （DCD） Group）， Group D （XCD Group） and Group E （dexamethasone Group）， with 10 rats in each group. Asthma model was induced by OVA and the factors of phlegm－heat were applied to establish the model. After the model was established successfully， Groups C， D， and E were given DCD （0.067 g/mL）， SCD （0.036 g/mL）， and dexamethasone solution （0.05 mg/100 g/d） at a dose of 1.5 mL/100 g， respectively； while groups A and B were given physiological saline in equal proportion once a day. After 14 days of continuous medication， the general conditions of rats in each group were observed. The pathological changes of the lung tissue were observed by HE staining， and the contents of IL－6， transforming growth factor－β（TGF－β）， IL－17 and IL－10 in broncho－alveolar lavage fluid were compared by enzyme－linked immunosorbent assay （ELISA）. Results：Pathological section results showed that no inflammatory cell infiltration was observed in group A， alveolar dilation was good， and bronchial mucosa was normal. A large number of eosinophilic granulocyte and lymphocyte infiltration were found in group B. The inflammatory infiltration of cells in groups C， D and E were reduced， and the pathological conditions were improved. ELISA results showed that compared with group A，the levels of IL－6 and IL－17 in alveolar lavage fluid in group B significantly increased （all P<0.01）.Compared with group B， IL－6 and IL－17 levels in groups C， D and E decreased （all P < 0.01）. Compared with group A， the levels of TGF－β and IL－10 in alveolar lavage fluid in group B decreased （all P < 0.01）.Compared with group B， the levels of two cytokines in group D significantly increased （all P < 0.01）. The level of TGF－β in group C increased （P < 0.05）， and the level of IL－10 significantly increased （P < 0.01）. The level of IL－10 in group E significantly increased （P < 0.01）， but the level of TGF－β did not significantly increase （P > 0.05）. Conclusion： Xuanbai Chengqi Decoction can treat asthma of phlegm heat and fu－organ excess type through the method of discharge heat and unblocking fu－organs， improving asthma symptoms， alleviating pathological damage to rat lung tissue， and changing the contents of IL－6， TGF－β，IL－17， and IL－10 in alveolar lavage fluid， thus， regulating the imbalance of Th17/Treg.

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